PlasMapper 3.0

Generate and Annotate Publication Quality Plasmid Maps Online

FASTA or raw DNA sequence. Non-ACTG inputs will be ignored. Sequence length must be between 21 and 20,000.


Try an example plasmid:

Welcome to PlasMapper 3.0!

PlasMapper 3.0 is a web server that allows users to generate, edit, annotate and interactively visualize publication quality plasmid maps. Plasmid maps are used ubiquitously in molecular biology and they provide a simple, visual approach to plan, design, share and publish critical information about cloning experiments. PlasMapper 3.0 has provided access to new and extended plasmid databases from which users can pick a wide variety of plasmid. Additionally, it offers an option of automated codon optimization and BLAST sequence alignment. PlasMapper 3.0 supports a variety of input formats including raw sequence, FASTA files and its own PlasMapper JSON format.

Please cite:

1. PlasMapper 3.0:
Wishart, D. S., Ren, L., Leong-Sit, J., Saha, S., Grant, J. R., Stothard, P., Singh, U., Kropielnicki, A., Oler, E., Peters, H., Gautam, V. (2023), PlasMapper 3.0 - A Web Server for Generating, Editing, Annotating and Visualizing Publication Quality Plasmid Maps, Nucleic Acids Research,

2. PlasMapper 2.0:
Dong, X., Stothard, P., Forsythe, I. J., & Wishart, D. S. (2004). PlasMapper: a web server for drawing and auto-annotating plasmid maps. Nucleic Acids Research, 32(Web Server Issue), W660.

Please direct any questions or feedback here
PlasMapper 3.0 is a project made for the Wishart Lab at the University of Alberta.

This project is supported by the Canadian Institutes of Health Research, Canada Foundation for Innovation, and by The Metabolomics Innovation Centre (TMIC), a nationally-funded research and core facility that supports a wide range of cutting-edge metabolomic studies. TMIC is funded by Genome Canada and Genome Alberta, a not-for-profit organization that is leading Canada's national genomics strategy with $900 million in funding from the federal government.