About PlasMapper 3.0

PlasMapper 3.0 is a web server that allows users to generate, edit, annotate and interactively visualize publication quality plasmid maps. It is the successor to PlasMapper 2.0 and offers many features found only in commercial plasmid mapping/editing packages. PlasMapper 3.0 allows users to paste or upload plasmid sequences as input or to upload existing plasmid maps from its large database of > 2000 pre-annotated plasmids (PlasMapDB). This database can be searched by plasmid names, sequence features, restriction sites, preferred host organism, and sequence length. PlasMapper 3.0 also supports the annotation of new or never-before-seen plasmids using its own feature database (FeatureDB) that contains common promoters, terminators, regulatory sequences, replication origins, selectable markers and other features found in most cloning vectors and plasmids. PlasMapper 3.0 has several interactive text editors/viewers that allow users to select and view plasmid regions, insert genes, modify restriction sites or perform codon optimization. The graphics for PlasMapper 3.0 have also been substantially upgraded over what was in PlasMapper 2.0. Specifically, PlasMapper 3.0 now offers an interactive, full-color plasmid viewer/editor that allows users to zoom, rotate, re-color, linearize, circularize, edit annotated features and modify the image or labels to improve the esthetic qualities of their plasmid map and textual displays. All the plasmid images and textual displays are downloadable in multiple high-resolution formats.

PlasMapper 3.0 was conceived, designed, developed, tested and supported by Leah Ren, Jacques Leong-Sit, Sukanta Saha, Jason R. Grant, Paul Stothard, Upasana Singh, Abby Kropilenicki, Eponine Oler, Harrison Peters, Vasuk Gautam and David S. Wishart.

Wishart, D. S., Ren, L., Leong-Sit, J., Saha, S., Grant, J. R., Stothard, P., Singh, U., Kropielnicki, A., Oler, E., Peters, H., Gautam, V. (2023), PlasMapper 3.0 - A Web Server for Generating, Editing, Annotating and Visualizing Publication Quality Plasmid Maps, Nucleic Acids Research, https://doi.org/10.1093/NAR/GKAD276

Source Code:
Please access the PlasMapper source code and instructions for installation here (BitBucket).

Please direct any questions or feedback here
PlasMapper 3.0 is a project made for the Wishart Lab at the University of Alberta.

This project is supported by the Canadian Institutes of Health Research, Canada Foundation for Innovation, and by The Metabolomics Innovation Centre (TMIC), a nationally-funded research and core facility that supports a wide range of cutting-edge metabolomic studies. TMIC is funded by Genome Canada and Genome Alberta, a not-for-profit organization that is leading Canada's national genomics strategy with $900 million in funding from the federal government.